What is a Single-Domain Antibody?
Single-Domain antibodies (sdAbs), also known as Nanobodies, are small antigen-binding fragments (~15 kDa) that are derived from heavy chain only antibodies present in camelids (VHH, from camelids), and cartiaginous fishes (VNAR, from sharks).
Advantages of sdAbs
- Small size, molecular weight of only ~15 KDa, than conventional antibody ~150 Kda
- Enhanced tissue penetration, can cross the blood-brain barrier
- Unique binding capacity to small cavities or clefts
- High affinity and specificity
- Highly stable under extreme temperature and pH
- High solubility, great imaging agents due to rapid clearance in vivo
- Can be readily selected and produced in bacterial and yeasts
- Cost-effective, virtually unlimited supply at constant high quality without batch-to-tach variations.
Our Services
We carry out sdAbs development through both Immune and Naïve phage libraries.
- Customized library construction services under M13 filamentous phage display system
- A large number of animals available, established and optimized workflow for camel, alpaca and shark immunization
- Professional animal handling and caring
- Big capacity: capable of carrying out multiple projects at the same time
- Flexible schedule: we can start the project once antigen is ready
- Strong specificity: we are able to target proteins with 1-10 amino acids differences
- Small and large scale expression of proteins in E.coli and Pichia Pastoris
workflow
