Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies

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Monoclonal antibodies are among the most significant biological tools used in medicine and biology that
have revolutionized the field of diagnostics, therapeutics, and targeted drug delivery systems for many
diseases. Among them, rabbit monoclonal antibodies have attracted significant attention for having high
affinity and specificity. During the past few decades, different techniques have been developed to produce
monoclonal antibodies. Single B cell cloning technology offers many advantages compared to other
methods and has been used to generate monoclonal antibodies from different species including rabbits.
This review briefly describes some of these methods, with main focus on single B cell cloning and
production of rabbit monoclonal antibodies.

Comparison between monoclonal and polyclonal antibodies
Diagram summarizing the generation of antigen-specific rabbit monoclonal antibodies by single B cell
cloning. PBMCs peripheral blood mononuclear cells, FACS fluorescence-activated cell sorting, RT-PCR reverse
transcription polymerase chain reaction, VH variable domain of heavy chain, VL variable domain of light chain,
LS leader sequence, HCD heavy-chain constant domain, LCD light-chain constant domain, HC heavy chain, LC
light chain, ELISA enzyme-linked immunosorbent assay

Ref: Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies. Book: Genotype Phenotype Coupling pp 423-441

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